Journal: International Journal of Molecular Sciences

Article Title: Modulation of ESKAPE Bacteria Properties by NK-92 and NK-92-Derived LEVs: First Insights

doi: 10.3390/ijms27093953

Figure Lengend Snippet: Effect of NK-92 cells on E. faecium colony formation and growth. Colony formation assay: E. faecium plated alone (Control) or co-cultured with NK-92 cells (Treated). ( Left ) Raw plate images; ( Right ) automated analysis (Scan500, Interscience, Saint Nom la Bretèche, France). T 0 /T 1 : plating time points, E + 0n = 10 n .

Article Snippet: Also, we used ESKAPE group bacteria: Enterococcus faecium (19434), Staphylococcus aureus (29213), Klebsiella pneumoniae (13883), Acinetobacter baumannii (19606), Pseudomonas aeruginosa (27853) and Enterobacter spp. (13047) (ATCC, USA) and cultured them on agarose medium, under appropriate biosafety containment, in accordance with institutional safety protocols for handling pathogenic microorganisms.

Techniques: Colony Assay, Control, Cell Culture

Journal: Frontiers in Microbiology

Article Title: Pentocin MQ1: A Novel, Broad-Spectrum, Pore-Forming Bacteriocin From Lactobacillus pentosus CS2 With Quorum Sensing Regulatory Mechanism and Biopreservative Potential

doi: 10.3389/fmicb.2018.00564

Figure Lengend Snippet: Antibacterial Spectrum of pentocin MQ1.

Article Snippet: It was also inhibitory albeit to a less extent toward S. pyogenes ATCC 12344, S. aureus RF122, P. aeruginosa PA7, E. faecium ATCC 19434, E. faecium ATCC 27270, E. faecium ATCC 27273, E. faecium ATCC BAA-2318, E. faecium ATCC BAA-2127, E. faecium ATCC 6569, E. faecium ATCC 25307, and E. faecium ATCC 349 but was not active against S. mutans GEJ11, L. lactis ATCC 11454 and Corynebacterium spp.

Techniques: Inhibition

Journal: International journal of food sciences and nutrition

Article Title: The use of Lactobacillus plantarum esterase genes: a biotechnological strategy to increase the bioavailability of dietary phenolic compounds in lactic acid bacteria.

doi: 10.1080/09637486.2021.1900078

Figure Lengend Snippet: Figure 1. Biochemical pathway followed by L. plantarum for the transformation of (A) hydroxybenzoic and (B) hydroxycinnamic acid derivatives. Some of the genes and enzymes involved are also indicated.

Article Snippet: The search revealed that a high number of Enterococcus faecium strains possessed a protein 73.88% identical to L. plantarum LpdC (accession Q3Y2U5 from E. faecium ATCC BAA-472).

Techniques: Transformation Assay

Journal: Journal of medicinal chemistry

Article Title: Hydroxybiphenylamide GroEL/ES inhibitors are potent antibacterials against planktonic and biofilm forms of Staphylococcus aureus .

doi: 10.1021/acs.jmedchem.8b01293

Figure Lengend Snippet: A. Chemical structure of the initial GroEL/ES hit inhibitor, 1, which was previously reported to potently inhibit the proliferation of E. faecium (EC50 = 0.15 μM) and S. aureus (EC50 = 0.20 μM).31 Analogs of inhibitor 1 have been synthesized and evaluated in this study, where the R1 through R5 substituents and substructures have been systematically removed to probe for the contributions that each make to inhibiting chaperonin system biochemical functions and bacterial and human cell viabilities. B. Chemical structures of related compounds used as anthelmintics in veterinary medicine.

Article Snippet: The ESKAPE bacteria were purchased from the American Type Culture Collection (ATCC): E. faecium (Orla-Jensen) Schleifer and Kilpper-Balz strain NCTC 7171 (ATCC 19434); S. aureus subsp. aureus Rosenbach strain Seattle 1945 (ATCC 25923); Multi-drug resistant S. aureus (MRSA) subsp. aureus Rosenbach strain HPV107 (ATCC BAA-44); K. pneumonia , subsp. pneumoniae (Schroeter) Trevisan strain NCTC 9633 (ATCC 13883); A. baumannii Bouvet and Grimont strain 2208 (ATCC 19606); P. aeruginosa (Schroeter) Migula strain NCTC 10332 (ATCC 10145); E. cloacae , subsp. cloacae (Jordan) Hormaeche and Edwards strain CDC 442–68 (ATCC 13047).

Techniques: Synthesized

Journal: Journal of medicinal chemistry

Article Title: Hydroxybiphenylamide GroEL/ES inhibitors are potent antibacterials against planktonic and biofilm forms of Staphylococcus aureus .

doi: 10.1021/acs.jmedchem.8b01293

Figure Lengend Snippet: Correlation plots comparing IC50 values for compounds tested in the GroEL/ES-dMDH refolding assay with EC50 values for inhibiting E. faecium (A) and MRSA (B) proliferation. While a general trend is observed between inhibiting the GroEL/ES chaperonin system and E. faecium proliferation (Spearman correlation coefficient comparing log(I/EC50) values in each assay is 0.9628, p < 0.0001), supporting on-target effects in bacteria, inhibitors are more potent against MRSA (Spearman correlation coefficient comparing log(I/EC50) values in each assay is 0.8042, p < 0.0001), suggesting potential off-target effects and/or greater GroEL/ES sensitivity in S. aureus bacteria. Results plotted in the grey zones represent IC50 and EC50 values higher than the maximum concentrations listed.

Article Snippet: The ESKAPE bacteria were purchased from the American Type Culture Collection (ATCC): E. faecium (Orla-Jensen) Schleifer and Kilpper-Balz strain NCTC 7171 (ATCC 19434); S. aureus subsp. aureus Rosenbach strain Seattle 1945 (ATCC 25923); Multi-drug resistant S. aureus (MRSA) subsp. aureus Rosenbach strain HPV107 (ATCC BAA-44); K. pneumonia , subsp. pneumoniae (Schroeter) Trevisan strain NCTC 9633 (ATCC 13883); A. baumannii Bouvet and Grimont strain 2208 (ATCC 19606); P. aeruginosa (Schroeter) Migula strain NCTC 10332 (ATCC 10145); E. cloacae , subsp. cloacae (Jordan) Hormaeche and Edwards strain CDC 442–68 (ATCC 13047).

Techniques: Bacteria